Figure 5.

Impact of pFAM21805 on biofilm formation of K-12 MG1655. Biofilm formation of E. coli K-12 MG1655 wild-type and K-12 MG1655 transconjugants harboring pFAM21805, pFAM21805 ΔorfE (deletion of LHR2-encoded putative di-guanyl cyclase), or pFAM21805 Δmrk (deletion of type 3 fimbriae genes). Bacteria were grown in ABTCAA media for 48 h at 12, 28, and 37°C, after which biofilm formation was quantified by crystal violet (CV) staining. Bars represent averages and standard deviations (^*p < 0.05, ^**p < 0.001, tested by 1-way ANOVA vs. K-12 wt control, α = 0.05, Holm-Sidak).