Table 1.
E. coli strains used in this study.
| Strain | Misc.a | Origin | References |
|---|---|---|---|
| FAM21805 | Heat resistant; harbors LHR1 and LHR2 | Swiss raw milk cheese | Peng et al., 2012, 2013a; Marti et al., 2016 |
| FAM21805 ΔLHR1 | Knock-out mutant, LHR1 (orf2-orf16) replaced with kanr | This study | |
| FAM21805 ΔLHR2 | Knock-out mutant, LHR2 (orf2-orfF) replaced with kanr | This study | |
| FAM21805 ΔLHR1&2 | Knock-out mutant, LHR1&2 replaced with kanr | This study | |
| FAM21805 Δmrk | Knock-out mutant, mrkABCDF replaced with tetr | This study | |
| FAM21805 ΔorfE | Knock-out mutant, orfE replaced with tetr | This study | |
| FAM21805 LHR1 kanr | LHR1 tagged with kanr without knock-out of known ORFs; donor of LHR1 in HGT experiments | This study | |
| FAM21805 LHR2 tetr | LHR2 tagged with tetr without knock-out of known ORFs; donor of LHR2 in HGT experiments | This study | |
| C604-10 | Heat resistant, ESBL-producing | Human clinical isolate | Olesen et al., 2013; Boll et al., 2016 |
| C604-10 LHR1 kanr | LHR1 tagged with kanr without knock-out of known ORFs; donor of LHR1 in HGT experiments | Boll et al., 2016 | |
| C604-10 LHR2 tetr | LHR2 tagged with tetr without knock-out of known ORFs; donor of LHR2 in HGT experiments | Boll et al., 2016 | |
| MG1655 nalr rifr | Recipient in HGT experiments, laboratory strain | Commensal K-12 strain | Møller et al., 2003 |
| MG1655 LHR2 (21805) | MG1655 transconjugant harboring LHR2 plasmid of FAM21805 tagged with tetr without knock-out of known ORFs | This study | |
| MG1655 LHR1&2 (21805) | MG1655 transconjugant harboring LHR1&2 of FAM21805; LHR1 tagged with kanr without knock-out of known ORFs | This study | |
| MG1655 LHR2 ΔorfE (21805) | MG1655 transconjugant harboring LHR2 plasmid of FAM21805 tagged by replacing a putative di-guanyl cyclase (orfE) with tetr | This study | |
| MG1655 LHR2 Δmrk (21805) | MG1655 transconjugant harboring LHR2 plasmid of FAM21805 tagged by replacing mrkABCDF with tetr | This study | |
| MG1655 LHR1 (C604-10) | MG1655 transconjugant harboring LHR1 of C604-10; tagged with kanr without knock-out of known ORFs | This study | |
| MG1655 LHR2 (C604-10) | MG1655 transconjugant harboring LHR2 of C604-10 tagged with tetr without knock-out of known ORFs | This study | |
| FAM21843 | Heat resistant; harbors LHR1 | Swiss raw milk cheese | Peng et al., 2012, 2013a; Marti et al., 2016 |
| FAM22873 | eaeA (intimin) +, stx1+, serotype O26+ | Swiss raw milk | This study |
| FAM22873 LHR2 (21805) | FAM22873 transconjugant harboring LHR2 plasmid of FAM21805 tagged with tetr without knock-out of known ORFs | This study | |
| FAM22873 ΔLHR2 (21805) | FAM22873 transconjugant harboring LHR2 plasmid of FAM21805 tagged by replacement of LHR with kanr | This study | |
| FAM23288 | stx2+ | Swiss raw milk | This study |
| FAM23288 LHR2 (21805) | FAM23288 transconjugant harboring LHR2 plasmid of FAM21805 tagged with tetr without knock-out of known ORFs | This study | |
| FAM23288 ΔLHR2 (21805) | FAM23288 transconjugant harboring LHR2 plasmid of FAM21805 tagged by replacement of LHR with kanr | This study | |
| 55989 | Enteroaggregative E. coli (EAEC) strain | Human clinical isolate | Bernier et al., 2002 |
| 55989 LHR2 (21805) | 55989 transconjugant harboring LHR2 plasmid of FAM21805 tagged with tetr without knock-out of known ORFs | This study | |
| 55989 ΔLHR2 (21805) | 55989 transconjugant harboring LHR2 plasmid of FAM21805 tagged by replacement of LHR with kanr | This study | |
| C227-11 φcu | Shiga toxin-producing EAEC strain cured of the Stx2a-bearing phage | Human clinical isolate | Zangari et al., 2013 |
| C227-11 φcu LHR2 (21805) | C227-11 φcu transconjugant harboring LHR2 plasmid of FAM21805 tagged with tetr without knock-out of known ORFs | This study | |
| C227-11 φcu ΔLHR2 (21805) | C227-11 φcu transconjugant harboring LHR2 plasmid of FAM21805 tagged by replacement of LHR with kanr | This study. |
a
kanr, kanamycin resistance cassette; tetr, tetracycline resistance cassette; nalr, nalidixic acid resistant; rifr, rifampicin resistant.