FIGURE 2.
ALA1 acts through both RDR1/6-related and -unrelated pathways to mediate antiviral immunity. (A) Phenotypes of rdr1 rdr6, ala1-2 rdr6, ala1-2 rdr1 and ala1-2 rdr1 rdr6 at 21 days after infection with mock or CMV2aTΔ2b. (B) Phenotypes of Col-0, rdr1 rdr6, ala1-2 rdr6 and ala1-2 rdr1 rdr6 at 45 days after infection with mock or CMV2aTΔ2b. (C) Immunoblotting analysis to detect the accumulations of CMV2aTΔ2b from leaves of Col-0, rdr6, rdr1, ala1-2, ala1-2 rdr6, ala1-2 rdr1, ala1-2 rdr1 rdr6 and rdr1 rdr6 at 21 days after infection with mock or CMV2aTΔ2b. The coat protein (CP) of CMV2aTΔ2b was detected by anti-CP (α-CP) antibody. The large subunit of ribulose-1,5-bisphosphate was used as the loading control.