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ALA1 is involved in gene silencing. The effect of ala1-2 on post-transcriptional silencing of 35S::GUS transgene of the L1 line. The 35S::GUS transgene was post-transcriptionally silenced in the L1 line (Boutet et al., 2003); the homozygous ala1-2 mutation prevents silencing of the GUS transgene in all expanded rosette leaves of the L1 line (ala1 35S::GUS), which was identified from F2 population of ala1-2 and L1.
