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. 2017 Apr 7;8:422. doi: 10.3389/fpls.2017.00422

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Copyright © 2017 Zhu, Gao, Xu, Wu, Song, Jiang, Zhu, Qi and Xie.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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ALA2 acts additively with RDR6 and redundantly with ALA1 in antiviral immunity. (A) Phenotypes of enor2 rdr6, and the ala2-crispr1 mutant generated by CRISPR/Cas9 in rdr6 (ala2-crispr1 rdr6) at 21 days after infection with mock or CMV2aTΔ2b. (B) Immunoblotting analysis to detect the accumulations of CMV2aTΔ2b from leaves of enor1 rdr6 and enor2 rdr6 at 21 days after infection with mock or CMV2aTΔ2b. The coat protein (CP) of CMV2aTΔ2b was detected by anti-CP (α-CP) antibody. The large subunit of ribulose-1,5-bisphosphate was used as the loading control. (C) Phenotypes of 30-day-old seedlings of rdr6, enor1 rdr6, enor2 rdr6, and enor1 enor2 rdr6.