Figure 3.

The T. gondii-experienced CD4+ and CD8+ T cells are programmed to produce inflammatory mediators during sepsis. Naïve or chronically T. gondii-infected mice were subjected to CLP to evaluate the frequencies of IFN-γ- or TNF-α-producing CD4⁺ and CD8⁺ T cells in the spleen (A–D). Peritoneal leukocytes were recovered from the peritoneal lavage 24 h after CLP, then stimulated with PMA-ionomycin, and stained for flow cytometry analysis (E–J). Data are presented as the means ± SEM for 4 animals in three different experiments. Statistical analysis was performed using ANOVA followed by Tukey's test; ^*p < 0.05; ^**p < 0.01; ^***p < 0.001.