Figure 5.

Restoration of selected target genes in Ets1^−/− B cells. (A) Western blot analysis of Ptpn22 [with lysates from unsorted virally infected wild-type (W) and knockout (K) B cells] and Stat4 expression [with lysates from the packaging cell line (PLAT-E) or virally infected B cells (B cells); note that the small text under the Stat4 blot represents Stat4 expression levels normalized to GAPDH]. (B) Green fluorescent protein (GFP) profiles of virally infected cells. Red shaded curves are background staining of control non-infected cells, while open black curves are GFP staining in virally infected cells. Bold red type is the mean fluorescent intensity of GFP in the GFP⁺ gated population. (C) Analysis of GFP⁺ WT and Ets1^−/− B cells to show differentiation to B220-low CD138⁺ plasmablasts (boxed). (D) Mean ± SEM of the percentage of plasmablasts within the GFP⁺ population in three independent viral infection experiments.