Table 4.

Nucleotide sequences of primers specific to L-theanine metabolic related genes and CsTBP gene used for qRT-PCR.

Gene	Forward primer 5′–3′	Reverse primer 5′–3′
TS1	AGACCGCCGACATCAACAC	ATGGCTTCCACAGCAGAGT
TS2	CCTAAACCTATTGAGGG TGACTG	TCCTGTAAGCCGACGCTCATT
GS1	ATCAGTTGTGGATGGCTCG	CACTTCGCATGGACTTGGTAC
GS2	GTGGCACCAACGGAGAAGT	CAAGGATGTATCTAGCGCACC
NiR	GGACAGGCTGCCCAAATAG	TCACTCCCAATCCTCCCTC
NR	TTGATGCTTGGGCTGACA	ACGGACCAGGGATGTGCT
GGT1	GATGAATCTTGGTGATCCTGAT	TTCCACCGTCCACCATAGT
GGT3	GGAGTCAGCTTCAAGATCACG	CAGTAGGCGTCGAGAAGTCAC
GDH1	GAGCTGAAGACATACATGACCA	GCACGAGCAACACGATTAA
GDH2	ATGTGGGACGAAGAGAAGGTG	GCAACACGATTCACTCCCAG
ALT	CGAGTCCTACGAGTCTTAT TATGC	GGAGGCGTTGACAATAGAATG
CuAO	CAGGTTGTTGAGGTGAATGTTA	AATCCAGTGGCGAGCAGA
PAO	GTCGGGGTGACGATACCTTAG	CACCACTTAGCGTCGACATTAT
GOGAT-Fe	TGCTGGTATGACTGGAGGTT	CAACTGCCAGAATAGCGGTA
GOGAT- NAD(P)H	GCAGCGAGGAGATGATTGA	CACCTTCCACATTGGTTGAG
SAMDC	TTCCAGCCAAGCGAGTTC	AACCTCCCTCCTTGCCGA
ADC	GGGCTTATGAGGAGGCAC	GCAAGAGGGTCCTGGCAT
CsTBP	GGCGGATCAAGTGTTGGAA GGGAG	ACGCTTGGGATTGTATTCGG CATTA