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. 2017 Apr 7;8:359. doi: 10.3389/fimmu.2017.00359

Figure 4.

Figure 4

Expression of surface marker CD40 in pulmonary antigen-presenting cells upon uptake of liposomes (LIPO) and virosomes (VIRO). Lung compartments (BAL, broncho-alveolar lavage; T, trachea; LP, lung parenchyma) were harvested 24 h after intranasal administration of empty liposomes or virosomes (“no OVA”) or with liposomes and virosomes coupled to OVA (“coupled OVA”) or PBS control (not shown). Particle negative (particle−) and particle positive (particle+) cell populations were analyzed for expression of surface marker CD40 measured by flow cytometry and frequency (%) is shown. Data represent five independent experiments. Statistical significance was determined by ANOVA followed by Tukey’s honest significant difference post hoc test to investigate individual paired comparisons (*p < 0.05; **p < 0.01; ***p < 0.001).