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. 2017 Apr 7;8:359. doi: 10.3389/fimmu.2017.00359

Figure 5.

Figure 5

Expression of surface marker CD80 in pulmonary antigen-presenting cells upon uptake of liposomes (LIPO) and virosomes (VIRO). Lung compartments (BAL, broncho-alveolar lavage; T, trachea; LP, lung parenchyma; LDLN, lung-draining lymph nodes) were harvested 24 h after intranasal administration of empty liposomes or virosomes (“no OVA”) or with liposomes and virosomes coupled to OVA (“coupled OVA”) or PBS control (not shown). Particle negative (particle−) and particle positive (particle+) cell populations were analyzed for expression of surface marker CD80 measured by flow cytometry and frequency (%) is shown. Data represent five independent experiments. Statistical significance was determined by ANOVA followed by Tukey’s honest significant difference post hoc test to investigate individual paired comparisons (***p < 0.001).