Fig. 2.

Intracellular growth and uptake of SidC-producing L. pneumophila Lp02 and Δpentuple. A, D. discoideum or (C) RAW 264.7 macrophages were infected (MOI 1) with L. pneumophila Lp02 or Δpentuple harboring pCR080 (DsRed, SidC) or not. At given time points the infected cells were lysed, and CFU were determined. Mean and S.D. of triplicates are shown; data are representatives of three independent experiments. B, D. discoideum or (D) RAW 264.7 macrophages were infected (MOI 50, 30 min) with L. pneumophila Lp02 or Δpentuple harboring plasmid pCR079 (GFP, SidC). The infected cells were analyzed by FACS to determine the uptake index (u.i.; product of number of infected cells above the gate threshold and fluorescence intensity). Data represent mean and standard deviation of three independent experiments.