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. 2017 Feb 9;16(4):622–641. doi: 10.1074/mcp.M116.063453

Table I. Strains and plasmids used in this study.

Strain/plasmid Relevant properties/description a Reference
L. pneumophila
    Lp02 L. pneumophila sg 1 Philadelphia-1, thyA, rpsL, hsdR (61)
    YAM10 (Δ2ab) L. pneumophila Lp02 Δlpg1104-lpg1128, Δlpg1136-lpg1169 (37)
    YAS4 (Δ3) L. pneumophila Lp02 Δlpg1603-lpg1686, tufBC197A (37)
    YAM35 (Δ4a) L. pneumophila Lp02 Δlpg1933-lpg1999 (37)
    YAS59 (Δ6a) L. pneumophila Lp02 Δlpg2369-lpg2465 (37)
    YAS64 (Δ7a) L. pneumophila Lp02 Δlpg2508-lpg2573 (37)
    YAM40 (Δpentuple) L. pneumophila Lp02 Δlpg1104-lpg1128, Δlpg1136-lpg1169, Δlpg1603-lpg1686, tufBC197A, Δlpg1933-lpg1999, Δlpg2369-lpg2465, Δlpg2508-lpg2573 (37)
    Lp053 L. pneumophila Lp02 ΔdotA053 (62)
Plasmids
    pCR079 pMMB207C-Ptac-RBS-gfp-RBS-MCS-sidC (21)
    pCR080 pMMB207C-Ptac-RBS-dsred-RBS-MCS-sidC (21)
    pGFP-Rap1 GFP-Rap1 (alias RapA) expression construct, pDM317 (40)
    pRalGDSRBD-GFP RalGDSRBD-GFP expression construct, pDM115 (40)
    pNT28 pMMB207C, gfp (constitutive) (63)
    pSW001 pMMB207C, dsRed-express (constitutive) (64)