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Changes of global levels of 5mC and 5hmC during erythropoiesis, and effects of TET2 or TET3 knockdown on global levels of 5mC. (A) 5mC levels of control, TET2, and TET3 knockdown erythroid cells at distinct developmental stages as measured by LC-MS. (B) 5hmC levels of CD34+ cells and erythroid cells at distinct developmental stages as measured by LC-MS. Results are from 3 independent replicates. *P < .05. LC-MS, liquid chromatography-tandem mass spectrometry.
