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. 2017 Apr 6;3:5. doi: 10.1186/s40851-017-0066-7

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Double-plotted actograms showing locomotor rhythms of crickets Gryllus bimaculatus. (a–j) Locomotor activity of DsRed2 ^RNAi (a), Gb’cry1 ^RNAi (b, c), Gb’cry2 ^RNAi (d–g), or Gb’cry1 ^RNAi and Gb’cry2 ^RNAi (h–j) crickets recorded under LD 12:12 and DD at a constant temperature of 25 °C. Arrowheads indicate the day when the crickets were transferred from LD to DD. Gb’cry1 ^RNAi crickets showed a locomotor rhythm free-running in DD with a period similar to that of DsRed2 ^RNAi crickets (a–c), but Gb’cry2 ^RNAi and Gb’cry1/Gb’cry2 double RNAi crickets showed variable free-running periods (d–j). For further explanations, see the text