Fig. 5.

Effects of Gb’cry1 ^RNAi (a), Gb’cry2 ^RNAi (b), and Gb’cry1 ^RNAi/Gb’cry2 ^RNAi (c) on daily clock gene expression. Relative abundance of Gb’per, Gb’tim, Gb’cry1, and Gb’cry2 mRNA in the optic lobes are shown for intact (blue) and Gb’cry1 ^RNAi (yellow) (a), Gb’cry2 ^RNAi (green) (b), and Gb’cry1 ^RNAi/Gb’cry2 ^RNAi (pink) adult male crickets (Gryllus bimaculatus) (c). The data for intact Gb’cry1 and Gb’cry2 mRNA are replotted from Fig. 2. In dsRNA-injected crickets, the optic lobes were collected about seven days after the dsRNA injection. The abundance of mRNA was measured by quantitative real-time RT-PCR with total RNA extracted from the optic lobes. The data collected from 3 and 5 independent experiments were averaged and plotted as mean ± SEM for dsRNA-injected and intact crickets, respectively. The abundance of Gb’rpl18a mRNA was used as an internal reference. Asterisks indicate significant difference between intact and treated crickets (*P < 0.05, **P < 0.01, t-test). Different letters indicate that values are significantly different with each other (ANOVA followed by Tukey-test, P < 0.05). For further explanations, see the text