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. 2017 Apr 6;3:5. doi: 10.1186/s40851-017-0066-7

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 6 — Suppressive effects of Gb’CRY1 or Gb’CRY2 proteins on tim transcription. Relative luciferase activity of tim-luc was measured in the presence of 100 ng of pAc-Clk with various combinations of the indicated pAc5.1B-Gb’cry1, pAc5.1B-Gb’cry2a, -b, -c, -d, -e, or -f expression plasmids. Signals were normalized to Rluc activity and then to the control signal of cells transfected with 100 ng of tim-luc and pAc-Clk. Error bars represent SEM (n = 3). A combination of Gb’cry2b and Gb’cry2c suppressed the transcription of E-box genes. Asterisks represents P < 0.01 versus control (Dunnett’s multiple range test)