Fig. 7.

Effects of RNAi of various clock genes on Gb’cry1 a and Gb’cry2 mRNA b in the cricket optic lobes. Adult male crickets (Gryllus bimaculatus) were treated with DsRed2 ^RNAi (gray), Gb’per ^RNAi (orange), Gb’tim ^RNAi (purple), Gb’Clk ^RNAi (blue), and Gb’cyc ^RNAi (light blue). The optic lobes were collected about 7 days after the dsRNA injection. The abundance of mRNA was measured by quantitative real-time RT-PCR with total RNA extracted from the optic lobes. The data collected from three and four independent experiments were averaged and plotted as mean ± SEM for clock gene dsRNA-injected and dsDsRed2-injected crickets, respectively. The abundance of Gb’rpl18a mRNA was used as an internal reference. The data for DsRed2 ^RNAi are replotted from Fig. 2. Asterisks indicate significant difference between DsRed2 ^RNAi crickets and those treated with clock gene dsRNAs: *P < 0.05, **P < 0.01, Dunnett’s test. Note that Gb’cry1 mRNA levels were never affected by RNAi of clock genes except for cyc ^RNAi at ZT22, and that Gb’cry2 mRNA levels showed a rhythmic expression after Gb’tim or Gb’cyc dsRNA treatments, but became arrhythmic when treated with Gb’per or Gb’Clk dsRNA. For further explanations, see the text