Table 1.

Primers used for qPCR, PCR, dsRNA synthesis, and cloning of coding regions for cell-based assay

Primers	Forward	Reverse
For qPCR
Gb’cry1	5′-AAAGCCAGTTACGCTGATGG-3′	5′-TGTAATCTCAGGCCATGTCG-3′
Gb’cry2	5′-AGCACCATCACACACTTCACA-3′	5′-ACACTCAGCGCAATCCACAC-3′
Gb’per	5′-AAGCAAGCAAGCATCCTCAT-3′	5′-CTGAGAAAGGAGGCCACAAG-3′
Gb’tim	5′-CCAAAGACAGGAAGCAGACC-3′	5′-GAATCCCAACACCAAAATGG-3′
Gb’rpl18a	5′-GCTCCGGATTACATCGTTGC-3′	5′-GCCAAATGCCGAAGTTCTTG-3′
For PCR
Gb’cry2-x2a	5′-GTGGTATCAACGCAGAAGTA-3′	5′-TCTAGGCACTGTAGTAGGAA-3′
Gb’cry2-x4	5′-TTCCGATGCATTTTCATCC-3′	5′-CGCCAAAGCTTCTGATTCTCC-3′
Gb’cry2-x10b	5′-CTCCTGAGAGTGTGCAACGT-3′	5′-ACTTGATGGAACTGCTGCCA-3′
For dsRNA synthesis
Gb’cry1#d1	5′-TAATACGACTCACTATAGGGGTTTCGACATGGCCTGAGAT-3′	5′-AATTAACCCTCACTAAAGGGCTTCTGGCGTTGGAAAATGT-3′
Gb’cry1#d2	5′-TAATACGACTCACTATAGGGTGGGAACAAGGAAAGACTGG-3′	5′-AATTAACCCTCACTAAAGGGCGACAATGAGGTGGAGGATT-3′
Gb’cry2#d1	5′-TAATACGACTCACTATAGGGCTGCGACAAATAACCCCAAC-3′	5′-AATTAACCCTCACTAAAGGGCTCTCAGGAGCATTCCAAGG-3′
Gb’cry2#d2	5′-TAATACGACTCACTATAGGGAAGCACACTGTGCATTGGTT-3′	5′-AATTAACCCTCACTAAAGGGCCGTTCTTTTCGATGATGCT-3′
Gb’per	5′-TAATACGACTCACTATAGGGCATTCATCGACTTCGTTCACC -3′	5′-AATTAACCCTCACTAAAGGGCTGAACGCCCAATCATGTCT -3′
Gb’tim	5′-AATTAACCCTCACTAAAGGGGTAAAGAAGATAGAGAGTAT-3′	5′-AATTAACCCTCACTAAAGGGTTGGAGAGAACTGAAGAGGT-3′
Gb’Clk	5′-TAATACGACTCACTATAGGGTACATCACGCCATAGCCTTG -3′	5′-AATTAACCCTCACTAAAGGGGGGATTGCTCTTCTTTGCTG -3′
Gb’cyc	5′-TAATACGACTCACTATAGGGCGTGCACTCGTACACTGAGG-3′	5′-AATTAACCCTCACTAAAGGGAGGTTCTGCTGCTTCTTTCG-3′
DsRed2	5’-TAATACGACTCACTATAGGGTCATCACCGAGTTCATGCG-3′	5′-TAATACGACTCACTATAGGGCTACAGGAACAGGTGGTGGC-3′
For cloning of coding region for cell-based assay
Gb’cry1	5′-ATGCAGGTACCAAGCCAGTTACGCTGATGGT-3′	5′-GGGCTCGAGAGTGGTCAAAGCAATTATCAT-3′
Gb’cry2#p1	5′-ATGCAGGTACCATGGGAAGCACACTTGCATT-3′	5′-ATGCAGCGGCCGCAATTGTGCTGTTGATTTAAAC-3′
Gb’cry2#p2	5′-ATGCAGGTACCAGTGCTCGTGTGTGTGTTTG-3′	5′-ATGCAGCGGCCGCAATTGTGCTGTTGATTTAAAC-3′

Gb’cry1, Gb’cry2#p1, Gb’cry2#p2 were used for cloning of Gb’cry1, Gb’cry2a ~ c, and Gb’cry2d ~ f, respectively