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Duration of detectability of Zika viral RNA in different specimens. qRT-PCR was performed using a commercial kit (PrimerDesign Ltd., UK) with serum, urine, saliva, and semen samples. A sample with a threshold cycle (CT) number ≤ 40 was considered to be Zika-positive. In patient 3, the final collection of semen was performed at 86th day after the symptom onset and the result was negative. It was not presented for the sake of brevity
