Table 1.
Strains used for ex vivo transcriptome comparisons
| α522 | MC58 | |
|---|---|---|
| Genome characteristics | ||
| GenBank accession number | FR845693 to FR845718 | AE002098 |
| No. of contigs in final assembly | 21 | 1 |
| Average single base coverage | 79-fold | 8.4-fold |
| Genome size (bp) | ≥2,074,170 | 2,272,360 |
| GC content (%) | 51.78% | 51.53% |
| Predicted number of coding sequences | ≥1985 | 2063 |
| Reference | This work | Tettelin et al. (2000) [123] |
| Molecular epidemiology | ||
| Source | Carrier | Patient |
| Country and year of isolation | Germany 2000 | United Kingdom 1983 |
| Sequence type | ST-35 | ST-74 |
| Clonal complex (CC) | ST-35 | ST-32 |
| Phylogenetic clade (PC) (a) | PC32/269 | PC32/269 |
| Frequency of CC in carriers(b) | 5.47% | 4.99% |
| Disease/carriage ratio(c) | 0.5 | 3.5 |
| Reference | Claus et al. (2005) [100] | McGuiness et al. (1991) [101] |
| Phenotypic characterization | ||
| Serum resistance (%)(d) | 117.7 ± 23.8 | 116.3 ± 15.7 |
| Adhesion to epithelial cells(e) | ||
| FaDu cells (%) | 10.7 ± 7.4 | 14.5 ± 8.5 |
| Detroit562 cells (%) | 8.9 ± 1.8 | 17.1 ± 5.3 |
| Invasion of epithelial cells(e) | ||
| FaDu cells (%) | 0.0008 ± 0.0001 | 0.0020 ± 0.0011 |
| Detroit562 cells (%) | 0.0011 ± 0.0004 | 0.0016 ± 0.0007 |
| In vitro logarithmic growth rates(f) | ||
| Rich medium (PPM+) (1/h) | 0.46 ± 0.01 | 0.47 ± 0.01 |
| Minimal medium (MMM) (1/h) | 0.06 ± 0.01 | 0.52 ± 0.02 |
| Ex vivo growth rates(g) | ||
| Saliva (1/min) | −0.041 ± 0.003 | −0.043 ± 0.003 |
| Blood (1/min) | 0.027 ± 0.004 | 0.023 ± 0.002 |
| CSF (1/min) | 0.010 ± 0.002 | 0.018 ± 0.003 |
(a) According to ref. [27, 28]
(b) According to ref. [100]
(c) According to ref. [2]
(d) Ratio in percent of viable bacteria after incubation for 30 min in the presence of 10% human serum and viable bacteria incubated without serum. Given are the average and standard deviation from four independent experiments with pooled human serum
(e) Ratio in percent of adherent and invasive bacteria, respectively, to total bacteria. Given are the average and standard deviation from at least four independent experiments
(f) Given are the mean and standard deviation of the logarithmic growth rate k according to log(OD 600(t)/OD 600(0)) = kt for t ∈ [1 h, 4 h] as depicted in Fig. 5 using linear regression (R 2 in vitro = 0.96 ± 0.07, p in vitro = 0.013 ± 0.026)
(g) Given are the mean and standard deviation of the growth rate k according to log(N(t)/N(0)) = kt for t ∈ [0 min, 120 min] as depicted in Fig. 5 using linear regression (R 2 ex vivo = 0.95 ± 0.05, p ex vivo = 0.018 ± 0.016)