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. 2017 Apr 7;7:46145. doi: 10.1038/srep46145

Table 2. D-serine potencies (IC 50 or EC 50) and efficacies (%) at GluD2-Lc D1–D2 hinge region and binding site mutants determined by two-electrode voltage clamp electrophysiology in Xenopus oocytes.

Protein IC50 (μM) [EC50 (μM) for GluN1/GluN2A] n D-serine efficacy (%) n
Mean 95% confidence interval Mean ± SEM
GluD2-Lc 154 138–173 11 46.2 ± 1.9 30
GluN1-1a (with GluN2A) 1.35 1.26–1.45 8 n/a*  
GluD2-Lc-(H)GluN1 1.19 0.84–1.69 6 12.9 ± 0.8 30
GluD2-Lc-(HS1)GluN1 129 100–165 9 29.1 ± 1.1 20
GluD2-Lc-(HS2)GluN1 n/a** n/a**   0.9 ± 0.4 20
GluD2-Lc(D542Y)-(HS2)GluN1 n/a** n/a**   –3.4 ± 0.5 20
GluD2-Lc(Y543Q)-(HS2)GluN1 10.5 8.5–13.0 8 24.1 ± 0.4 20
GluD2-Lc(S544G)-(HS2)GluN1 47.9 41.4–55.6 8 40.6 ± 0.5 20
GluD2-Lc(Y496F) 158 141–176 11 43.5 ± 2.0 20
GluD2-Lc(Y543Q) 81.5 73.4–90.4 11 36.2 ± 0.6 20
GluD2-Lc(A686S) 1020 911–1143 8 43.9 ± 0.7 20
GluD2-Lc(Y770F) 18.5 17.1–20.0 8 59.9 ± 1.7 20

*Efficacy cannot be calculated for an excitatory response.

**No response to D-serine.