Table 1. Bacteriophage MP1 structural proteins identified by ESI-MS/MS.
| Protein | Identified function | Band N° (most abundant) | Protein MW (Da) MS/MSa | Protein MW (Da)b | N° of unique peptides | Sequence coverage, % | HHPred significant match | E-valuec | Identitiy, %d |
|---|---|---|---|---|---|---|---|---|---|
| gp007 | — | 10 | 15,738 | 14,750 | 1 | 7.4 | — | — | — |
| gp012 | Exonuclease A | 7 | 28,702 | 26,030 | 2 | 9.9 | exonuclease A [Citrobacter phage Merlin] dCTP pyrophosphatase [Proteus phage vB_PmiM_Pm5461] | 1E-108 | 66% |
| gp016 | dCTP pyrophosphatase | 9 | 20,529 | 20,190 | 3 | 18.1 | 5E-91 | 72% | |
| gp018 | — | 7 | 33,158 | 32,490 | 5 | 22.9 | — | — | — |
| gp030 | RecA-like recombination | 5–7 (6) | 44,549 | 42,850 | 11 | 30.5 | RecA-like recombination protein [Serratia phage PS2] | 0.0 | 81% |
| gp038 | DNA polymerase accessory protein clamp loader subunit | 6–7 (6) | 36,767 | 36,470 | 3 | 10.8 | DNA polymerase accessory protein clamp loader subunit [Proteus phage vB_PmiM_Pm5461] | 1E-155 | 66% |
| gp039 | Sliding clamp | 7 | 26,260 | 25,380 | 5 | 21.3 | sliding clamp [Proteus phage vB_PmiM_Pm5461] | 4E-78 | 55% |
| gp042 | Hypothetical protein | 12 | 9,690 | 9,580 | 4 | 40.0 | hypothetical protein [Morganella morganii] | 2E-22 | 53% |
| gp049 | Hypothetical protein | 11 | 14,126 | 13,190 | 1 | 15.8 | hypothetical protein Pm5461_055 [Proteus phage vB_PmiM_Pm5461] | 7E-17 | 44% |
| gp051 | Sigma factor for late transcription | 9 | 21,122 | 20,500 | 4 | 26.4 | sigma factor for late transcription [Proteus phage vB_PmiM_Pm5461] | 7E-82 | 70% |
| gp059 | Hypothetical protein | 11 | 15,176 | 14,750 | 6 | 46.3 | hypothetical protein Aeh1p318 [Aeromonas phage Aeh1] | 3E-11 | 55% |
| gp069 | Thioredoxin | 7 | 40,600 | 39,370 | 2 | 6.6 | thioredoxin [Salmonella phage STML-198] | 0.0 | 80% |
| gp071 | Hypothetical protein | 10 | 19,622 | 17,150 | 4 | 29.8 | hypothetical protein Pm5461_072 [Proteus phage vB_PmiM_Pm5461] | 2E-24 | 39% |
| gp073 | — | 10–11 (10) | 20,635 | 19,200 | 8 | 44.3 | — | — | — |
| gp100 | Hypothetical protein | 11 | 17,714 | 16,480 | 3 | 17.0 | hypothetical protein Pm5461_100 [Proteus phage vB_PmiM_Pm5461] | 5E-42 | 60% |
| gp101 | Endolysin | 11 | 14,738 | 14,480 | 3 | 33.8 | peptidase M15 [Escherichia coli] | 3E-54 | 71% |
| gp113 | Hypothetical protein | 7-11 (8) | 23,179 | 22,310 | 6 | 35.3 | hypothetical protein Pm5461_106 [Proteus phage vB_PmiM_Pm5461] | 2E-42 | 43% |
| gp117 | Hypothetical protein | 10 | 16,842 | 16,160 | 4 | 28.9 | hypothetical protein VR7_gp147 [Enterobacteria phage vB_EcoM-VR7] | 9E-26 | 45% |
| gp119 | Hypothetical protein | 10 | 17,175 | 16,260 | 2 | 14.6 | hypothetical protein SP18_gp147 [Shigella phage SP18] | 5E-40 | 52% |
| gp136 | Hypothetical protein | 11 | 11,613 | 11,210 | 6 | 59.0 | hypothetical protein STP4a_129 [Salmonella phage STP4-a] | 2E-17 | 47% |
| gp137 | Hypothetical protein | 10 | 16,966 | 16,880 | 2 | 16.7 | gp57B conserved hypothetical protein [Enterobacteria phage T4] | 1E-79 | 77% |
| gp139 | dNMP kinase | 7 | 28,387 | 26,640 | 5 | 18.9 | dNMP kinase [Proteus phage vB_PmiM_Pm5461] | 1E-56 | 47% |
| gp140 | Tail completion and sheath stabilizer | 9 | 22,238 | 21,840 | 2 | 11.1 | gp3 tail completion and sheath stabilizer protein [Enterobacteria phage CC31] | 5E-80 | 58% |
| gp141 | DNA end protector | 7 | 31,464 | 30,950 | 3 | 9.2 | DNA end protector protein [Proteus phage vB_PmiM_Pm5461] | 4E-139 | 73% |
| gp147 | Baseplate wedge subunit | 2–3 | 72,921 | 72,370 | 18 | 37.9 | baseplate wedge subunit [Proteus phage vB_PmiM_Pm5461] | 0.0 | 72% |
| gp148 | Baseplate wedge subunit | 1 | 122,856 | 121,220 | 1 | 0,9 | baseplate wedge subunit [Proteus phage vB_PmiM_Pm5461] | 0.0 | 69% |
| gp149 | Baseplate wedge tail fiber connector | 5 | 39,290 | 38,630 | 2 | 7.4 | baseplate wedge tail fiber connector [Proteus phage vB_PmiM_Pm5461] | 0.0 | 77% |
| gp150 | Baseplate wedge subunit and tail pin | 7 | 31,824 | 31,340 | 2 | 6.3 | baseplate wedge subunit and tail pin [Proteus phage vB_PmiM_Pm5461] | 5E-135 | 67% |
| gp151 | Baseplate wedge subunit and tail pin | 2–3 | 67,569 | 67,110 | 14 | 33.9 | baseplate wedge subunit and tail pin [Citrobacter phage Moon] | 0.0 | 48% |
| gp152 | Baseplate wedge subunit and tail pin | 8 | 26,233 | 23,260 | 2 | 8,9 | baseplate wedge subunit and tail pin [Proteus phage vB_PmiM_Pm5461] | 3E-45 | 45% |
| gp153 | Short tail fibers | 5 | 48,108 | 47,610 | 9 | 27.8 | short tail fibers protein [Proteus phage vB_PmiM_Pm5461] | 3E-133 | 49% |
| gp158 | Tail sheath stabilization | 6 | 32,675 | 32,380 | 1 | 4.0 | tail sheath stabilization protein [Proteus phage vB_PmiM_Pm5461] | 8E-134 | 68% |
| gp161 | Tail sheath | 1–3,7 (3) | 73,206 | 72,620 | 26 | 51.3 | tail sheath protein [Proteus phage vB_PmiM_Pm5461] | 0.0 | 76% |
| gp162 | Tail tube | 9 | 20,855 | 18,570 | 4 | 21.3 | tail tube protein [Proteus phage vB_PmiM_Pm5461] | 3E-97 | 79% |
| gp163 | Portal vertex of head | 2–4 (3) | 61,044 | 60,300 | 16 | 41.0 | portal vertex of head [Proteus phage vB_PmiM_Pm5461] | 0.0 | 68% |
| gp165 | Prohead core | 11 | 17,074 | 16,190 | 1 | 6.9 | prohead core protein [Proteus phage vB_PmiM_Pm5461] | 3E-51 | 70% |
| gp166 | Prohead core scaffold and Protease | 10 | 24,248 | 23,130 | 3 | 16.1 | prohead core scaffold protein and protease [Proteus phage vB_PmiM_Pm5461] | 2E-111 | 79% |
| gp167 | Prohead core scaffold | 2–11 (6) | 32,335 | 30,360 | 17 | 77.9 | prohead core scaffold protein [Proteus phage vB_PmiM_Pm5461] | 3E-77 | 56% |
| gp168 | Major capsid | 1–11 (4) | 55,998 | 56,010 | 23 | 60.24 | major capsid protein [Proteus phage vB_PmiM_Pm5461] | 0.0 | 81% |
| gp169 | Head vertex | 4–6 (5) | 46,883 | 46,160 | 21 | 66.5 | head vertex protein [Proteus phage vB_PmiM_Pm5461] | 0.0 | 79% |
| gp187 | Baseplate tail tube initiator | 7 | 33,526 | 33,420 | 4 | 16.1 | baseplate tail tube initiator [Proteus phage vB_PmiM_Pm5461] | 7E-174 | 79% |
| gp189 | Baseplate hub subunit and tail length determinator | 2–3 | 63,213 | 62,730 | 2 | 4.4 | baseplate hub subunit and tail length determinator [Proteus phage vB_PmiM_Pm5461] | 3E-119 | 41% |
| gp190 | Baseplate hub distal subunit | 9 | 19,816 | 18,180 | 3 | 18.5 | baseplate hub distal subunit [Proteus phage vB_PmiM_Pm5461] | 3E-62 | 59% |
| gp191 | Baseplate hub subunit | 5 | 45,005 | 44,550 | 2 | 7.4 | baseplate hub subunit [Proteus phage vB_PmiM_Pm5461] | 0.0 | 68% |
| gp194 | Baseplate wedge subunit | 11 | 14,497 | 14,380 | 2 | 14.0 | baseplate wedge subunit [Proteus phage vB_PmiM_Pm5461] | 9E-56 | 66% |
| gp195 | recombination, repair and ssDNA binding protein | 10–11 | 17,680 | 15,740 | 3 | 22.4 | recombination, repair and ssDNA binding protein [Proteus phage vB_PmiM_Pm5461] | 3E-65 | 71% |
| gp201 | Head outer capsid | 5–6 (6) | 40,233 | 39,710 | 13 | 41.3 | head outer capsid protein [Proteus phage vB_PmiM_Pm5461] | 1E-176 | 68% |
| gp205 | RNA polymerase ADP-ribosylase | 1–3,5,7 (3) | 79,188 | 77,210 | 25 | 35.3 | RNA polymerase ADP-ribosylase [Proteus phage vB_PmiM_Pm5461] | 0.0 | 49% |
| gp206 | DNA ligase | 4,7 (7) | 55,737 | 55,740 | 10 | 27.4 | DNA ligase [Proteus phage vB_PmiM_Pm5461] | 0.0 | 61% |
| gp207 | Hypothetical protein | 8 | 24,524 | 24,410 | 4 | 21.6 | hypothetical protein Pm5461_192 [Proteus phage vB_PmiM_Pm5461] | 3E-126 | 84% |
| gp231 | RNA ligase 1 and tail fiber attachment catalyst | 5–7,10 (5) | 45,404 | 42,480 | 8 | 25.2 | RNA ligase 1 and tail fiber attachment catalyst [Proteus phage vB_PmiM_Pm5461] | 5E-147 | 58% |
| gp244 | Hypothetical protein | 10 | 16,419 | 16,420 | 2 | 15.7 | hypothetical protein Pm5461_225 [Proteus phage vB_PmiM_Pm5461] | 3E-11 | 31% |
| gp249 | dsDNA binding protein | 11 | 11,382 | 10,360 | 5 | 41.8 | dsDNA binding protein [Proteus phage vB_PmiM_Pm5461] | 8E-41 | 75% |
| gp251 | Long tail fiber proximal subunit | 1–3 (3) | 134,949 | 134,620 | 12 | 14.0 | long tail fiber proximal subunit [Proteus phage vB_PmiM_Pm5461] | 0.0 | 60% |
| gp253 | Long tail fiber distal connector | 10 | 19,223 | 17,300 | 3 | 30.7 | long tail fiber distal connector [Proteus phage vB_PmiM_Pm5461] | 2E-24 | 40% |
| gp254 | Long tail fiber distal subunit | 4 | 61,920 | 61,700 | 6 | 13.9 | long tail fiber distal subunit [Proteus phage vB_PmiM_Pm5461] | 2E-22 | 46% |
| gp266 | DNA topoisomerase | 4–5 (5) | 54,442 | 51,720 | 3 | 6.7 | DNA topoisomerase [Proteus phage vB_PmiM_Pm5461] | 0.0 | 63% |
| gp267 | Nucleoid disruption protein | 10 | 17,925 | 17,410 | 1 | 7.1 | nucleoid disruption protein [Proteus phage vB_PmiM_Pm5461] | 9E-25 | 44% |
aMolecular weight (MW) measured via mass spectrometry.
bMolecular weight (MW) predicted on the basis of amino acid sequence.
cHHpred E-value of the most significant matching protein model.
dHHpred percent sequence identity for a pairwise aa alignment with the template protein.
Identified phage proteins are listed below. Their mass and SDS-PAGE band have been indicated, as the number of identified peptides and the complete protein coverage %. It should be noted that Identifications with a low abundance of peptides (<4) could be the result of protein impurities remaining in the samples.