Table 2. Bacteriophage MP2 structural proteins identified by ESI-MS/MS.
| Protein | Identified function | Band N° (most abundant) | Protein MW (Da) MS/MSa | Protein MW (Da)b | N° of unique peptides | Sequence coverage, % | HHPred significant match | E-valuec | Identitiy, %d |
|---|---|---|---|---|---|---|---|---|---|
| gp06 | — | 12 | 12,086 | 11,550 | 2 | 25.2 | — | — | — |
| gp15 | Hypothetical protein | 7 | 22,670 | 21,720 | 1 | 4.5 | hypothetical protein MmP1_gp16 [Morganella phage MmP1] | 3E-48 | 53 |
| gp18 | Single-stranded DNA-binding protein | 6–8 (6) | 26,990 | 26,470 | 10 | 63.0 | single-stranded DNA-binding protein [Morganella phage MmP1] | 2E-133 | 87 |
| gp21 | Endolysin | 10 | 17,690 | 17,370 | 2 | 12.7 | lysozyme [Morganella phage MmP1] | 8E-102 | 93 |
| gp25 | Hypothetical protein | 11 | 8,294 | 7,830 | 1 | 21.6 | hypothetical protein MmP1_gp24 [Morganella phage MmP1] | 3E-24 | 93 |
| gp29 | — | 11–12 (11) | 13,080 | 10,520 | 4 | 38.5 | — | — | — |
| gp32 | Exonuclease | 5–6 | 36,568 | 35,060 | 5 | 19.4 | exonuclease [Morganella phage MmP1] | 0.0 | 90 |
| gp33 | Hypothetical protein | 12 | 9,793 | 9,360 | 1 | 15.1 | hypothetical protein MmP1_gp32 [Morganella phage MmP1] | 3E-17 | 74 |
| gp34 | Hypothetical protein | 10 | 8,919 | 8,440 | 4 | 59.0 | hypothetical protein MmP1_gp33 [Morganella phage MmP1] | 3E-23 | 70 |
| gp35 | Host Range Protein | 10 | 8,427 | 7,930 | 3 | 60.5 | hypothetical protein [Escherichia phage CICC 80001] | 2E-4 | 46 |
| gp36 | Head-to-tail joining protein | 1–5 (3) | 59,543 | 59,350 | 32 | 75.4 | head-to-tail joining protein [Morganella phage MmP1] | 0.0 | 92 |
| gp37 | Capsid assembly protein | 1–7 (5) | 38,204 | 35,430 | 15 | 56.9 | capsid assembly protein [Morganella phage MmP1] | 0.0 | 80 |
| gp38 | Major capsid protein | 1–12 (5) | 36,358 | 36,230 | 22 | 76.5 | major capsid protein [Morganella phage MmP1]; minor capsid protein [Morganella phage MmP1] | 0.0 | 89 |
| gp39 | Hypothetical protein | 1,4–7 (5) | 7,693 | 4,690 | 1 | 31.4 | hypothetical protein MmP1_gp37 [Morganella phage MmP1]; minor capsid protein [Morganella phage MmP1] | 7E-5 | 80 |
| gp40 | Tail tubular protein A | 7–8 (7) | 23,027 | 22,090 | 7 | 59.4 | tail tubular protein A [Morganella phage MmP1] | 6E-122 | 86 |
| gp41 | Tail tubular protein B | 1–5,7,10 (2) | 90,762 | 90,770 | 32 | 47.9 | tail tubular protein B [Morganella phage MmP1] | 0.0 | 88 |
| gp43 | Internal virio + n protein B | 8 | 21,669 | 20,410 | 10 | 50.5 | internal virion protein B [Morganella phage MmP1] | 1E-87 | 84 |
| gp44 | Internal virion protein C | 1–4,6–7,10 (3) | 82,793 | 82,570 | 38 | 54.4 | internal virion protein C [Morganella phage MmP1] | 0.0 | 76 |
| gp45 | Internal virion protein D | 1–7 (2) | 144,296 | 143,870 | 58 | 59.2 | internal virion protein D [Morganella phage MmP1] | 0.0 | 70 |
| gp46 | Tail fiber protein | 1–7,10,12 (3) | 63,591 | 63,260 | 31 | 64.5 | tail fiber protein [Morganella phage MmP1] | 0.0 | 72 |
| gp50 | DNA packaging protein B | 1,3 (3) | 68,739 | 66,550 | 7 | 11.9 | DNA maturase B [Morganella phage MmP1] | 0.0 | 92 |
aMolecular weight (MW) measured via mass spectrometry.
bMolecular weight (MW) predicted on the basis of amino acid sequence.
cHHpred E-value of the most significant matching protein model.
dHHpred percent sequence identity for a pairwise aa alignment with the template protein.
Identified phage proteins are listed below. Their mass and SDS-PAGE band have been indicated, as the number of identified peptides and the complete protein coverage %. It should be noted that Identifications with a low abundance of peptides (<4) could be the result of protein impurities remaining in the samples.