Figure 4.

Treatment with 3-MA or ATG7 knockdown attenuates inflammatory cytokine production in lung tissues or alveolar macrophages respectively. (a) Minipigs were subjected to lung ischemia and perfusion with pulmonary protective solution containing 3-MA (5 mM) or DMSO followed by reperfusion for 1 h or received sham operations (Sham). The levels of Il1b, Tnf, Il12 and Il6 mRNA expression in left lung tissue were detected by qPCR analysis. (b–g) Alveolar macrophages were transfected with the control siRNA, Atg7 siRNA or Becn1 siRNA. Sixty hour later, cells were treated with rpHMGB1 (1 μg/ml) (b, d and f) or rpHSP60 (1 μg/ml) (c, e and g) for the indicated periods. The levels of Il1b, Tnf, Il12 and Il6 mRNA expression were detected by qPCR analysis (b and c), or the levels of IL-1β and TNF in supernatants were measured by ELISA (d–g). Data are mean±S.E.M. of three individual experiments. **P<0.01