Fig. 1.

CPT-11 induces mastocytosis and alters S100β and HuC/D protein expression in small intestine. a Total and degranulated mast cells were count in the duodenum, jejunum, and ileum in ten microscope field (×400)/slide per mice from six mice in each group. Bars represent mean ± SEM of number of mast cell/field or percentage of degranulated mast cell per field. ^# p < 0.05 versus control group. b Representative images of western blot showing S100β and beta-actin (loading control) protein expression. Quantitative analysis indicated that CPT-11 increased S100β protein expression in the small intestine. c Representative images of western blot showing HuC/D and beta-actin (loading control) protein expression. Quantitative analysis indicated that CPT-11 decreased HuC/D protein expression in the small intestine. Bars represent mean ± SEM for six tissue samples in each group. ^# p < 0.05 versus control group. Student’s t test