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. 2017 Apr 7;7:45970. doi: 10.1038/srep45970

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Copyright © 2017, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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(a) 100 nm thin cross-section of an entire rat islet of Langerhans imaged at 2.5 nm pixel size (STEM). Full high-resolution data is available via nanotomy.org (see suppl. material). (b) Area of interest (indicated in a; cyan dot) shows parts of four cells with different granules based on grey levels and morphology. (c–f) Elemental content in the ROI as indicated. (g) Overlay of N (red), P (green) and S (blue) allows identification of cells and granules based on elemental content. (h) Overlay of back scatter ICD image (greyscale) over the color-image of g. Bars: 50 μm (a) and 2 μm (b–h). Maps of other elementals are available as Fig.S1.