. 2017 Apr 7;14:72. doi: 10.1186/s12985-017-0741-5

Table 1.

Statistics of next-generation sequencing of 30 avian paramyxovirus isolates in a single run

Data	Results
Cluster density (K/mm²)^a	917 +/- 19
Clusters passing filter^b	92.34%
Total number of reads	17762176
Pass-filter reads^c	16403251
Percentage of reads passing filter	96.31%
≥ Q30^d	77.9%
Lowest representation for any index^e	0.0007%
Highest representation for any index^e	7.16%

^a shows number of clusters per square millimeter (optimal cluster density is 1000–1200, can vary with chemistry)

^b indicates the purity of the signals detected from the clusters (i.e. signals passing chastity filter that is the ratio of the brightest base intensity divided by the sum of the brightest and second brightest base intensities and the filtration process removes the least reliable clusters from the image analysis results)

^c reads passing filter (about 15 million reads are expected from an optimally clustered flow cell)

^d percentage of bases with Phred quality score equal or greater to 30

^e percentage of pass-filter reads assigned to any index