Figure 2. Repeatability of profiling O-acetylation in sialic acids by methylamidation for Neu5NAc₂Hex₅HexNAc₄.

The graph shows the average relative intensities of OAc_0-6Hex₅HexNAc₄Neu5NAc₂, with error bars for standard deviation. (a) Five methylamidated samples were prepared from the pooled serum in the same day; (b) The day-to-day repeatability analysis was performed for three different days; (c) Native glycans were measured by ESI-MS.