Figure 1. Coherence changes in CA1 neurons during CFM consolidation.

(a,b) Experimental paradigm. Male C57BL/6J mice were recorded for a 24-h baseline period in their home cage, starting at lights-on, after which they either underwent single-trial CFC in a novel recording chamber (a) or sham training (b) (n=5 mice per group). Afterward, all mice were returned to their home cage for an additional 24 h of recording. Context-specific freezing was assessed as a measure of CFM consolidation 24 h later. (c,d) Coherent firing is shown for three representative FS interneurons (c) and three representative principal neurons from the same site (d) recorded in CA1 over the first 6 h of baseline (black) or the first 6 h following either CFC or sham conditioning (red or purple, respectively). Coherent firing is shown separately for periods of NREM, REM and wake. (e) Quantification of post-CFC changes in delta and theta coherence (from baseline) for FS interneurons and principal neurons. Box plots indicate 25th, 50th and 75th percentile values. ↑↓ indicates relative increases or decreases of P<0.05 for post CFC or post Sham versus baseline, Wilcoxon signed rank test. (f) Mean (±s.e.m.) frequency of SPWR events for CFC and Sham mice during the first 6 h of baseline recording, and following CFC or sham conditioning. ↑ indicates relative increase of P<0.05 for post CFC versus baseline, *indicates P<0.05 post CFC versus post Sham, Holm-Sidak post hoc test. (g) Mean (±s.e.m.) firing rate changes (from baseline values) during SPWR events. *indicates P<0.05 post CFC versus post Sham, Holm–Sidak post hoc test.