Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Apr 6;8:14773. doi: 10.1038/ncomms14773

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2017, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

PMC Copyright notice

(a) Sequences of peptides with modifications on CP2 (CP2.1, CP2.2 and CP2.3) used for cellular assays. (b,c) KDM4A stability is enhanced for CP2-treated cells in CETSA. (b) CETSA melting curves for Flag-KDM4A in U2OS cells with and without CP2(T13Z) treatment (0.5 μM). The actin T_m was 55 °C for treated and untreated cells. (c) Isothermal dose–response titration CETSAs of dosed U2OS cells at 55 °C demonstrate that both CP2 and CP2.3 stabilize KDM4A in a dose-dependent manner. Average±s.e.m. are shown (n>3, biological). Representative western blotting figures are shown.