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. 2017 Apr 6;8:14979. doi: 10.1038/ncomms14979

Figure 1. Biochemical and functional properties of metastatic 4T1 and non-invasive EMT6 murine tumours.

Figure 1

(a) Luciferase-expressing 4T1 and EMT6 murine mammary tumour cells generate same size tumours in syngeneic BALB/c mice. Scale bar, 1 cm. (b–d) 4T1 tumour-bearing animals develop spontaneous pulmonary metastasis and show early pulmonary infiltration and enlarged spleen compared to the EMT6 tumour-bearing animals. Scale bar, 100 μm (lung sections); and 1 cm (gross organ pictures). (e,f) 4T1 tumour cells produce higher levels of inflammatory cytokines compared to the non-metastatic EMT6 or 67NR as assessed by cytokine antibody array. (g) Mouse transcriptome analyses of 4T1 and EMT6 tumours grown in BALB/c mice reveal 781 differentially expressed genes. (h) Top common genes that are upregulated in both EMT6 and 4T1 tumours. (i) Select genes (implicated in metastatic process) that are distinctly upregulated in 4T1 tumour compared to the EMT6 are. Results are presented as mean±s.d. (n=3). *P<0.05, **P<0.005, unpaired t-test.