Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Apr 7;7:46269. doi: 10.1038/srep46269

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2017, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

PMC Copyright notice

(A) Western blot of the final products of RT-QuIC reaction seeded with brain homogenates or olfactory mucosa from sCJD, iCJD, FFI, AD, PD patients and healthy controls (HC). Samples were digested with PK [50 μg/mL] and immunoblot was probed with the C-terminal antibody SAF-84. Number in the right indicates the position of Mw. (B) Biochemical profile of amplified products (both brain homogenates and olfactory mucosa) was compared to that of FFI, sCJD-type 1 and sCJD-type 2 brain homogenates assessed before and after PMCA. FFI brain homogenate was concentrated 20-fold for PrP^Sc detection. The signal of PrP^Sc was assessed by means of Western blotting, after PK digestion, with the 6D11 antibody. Numbers in the right indicate the position of Mw: 21 kDa refers to type 1 PrP^Sc, while 19 kDa refers to type 2 PrP^Sc.