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. 2017 Apr 7;7:45740. doi: 10.1038/srep45740

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The stimulation with ethylene glycol during 60 days (ETG 60 D) and hydroxyproline during 60 days (HPL 60 D) increased the expression of TGF-β1 according to immunoblot image (A) and percentage of immunostaining area (C). There was an increase in the production of collagen type I (yellow to red tone) and collagen type III (greenish tone), showed by picrosirius red staining (E and G). Densitometric quantification of western blot bands (B), immunohistochemistry positive area (D) and collagen staining by picrosirius red (F and H) using ImageJ software. There was a significant increase in TGF-β1 protein expression and collagen production in HPL and ETG treated animals in comparison to controls (CTL) animals. Data are presented as means ± standard errors. N = 5 for each group. (*) Indicates significant differences compared with the control group at p < 0.05.