Table 2. Raw data of the activity of the 43 selected compounds originating Figure 2.
| Compound | Prolif raw | Prolif SE | Prolif Score | Prolif Total | Diff-CGT raw | Diff-CGT SE | Diff_CGT Score | Diff_CGT Total | Diff_MBP raw | Diff_MBP SE | Diff_MBP Score | Diff_MBP Total | TOTAL SCORE |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| PDGF | 3.17 | 0.98 | 10.00 | 31.75 | — | — | — | — | — | — | — | — | — |
| T3 + T4 | — | — | — | — | 4.21 | 0.49 | 3.00 | 12.63 | 8.95 | 1.98 | 5.00 | 44.75 | — |
| VULPINIC ACID | 2.11 | 0.59 | 10.00 | 21.08 | 11.19 | 4.49 | 3.00 | 33.58 | 60.00 | 54.54 | 5.00 | 300.00 | 354.65 |
| LOVASTATIN | 2.94 | 0.26 | 10.00 | 29.36 | 22.86 | 20.35 | 3.00 | 68.57 | 58.25 | 55.69 | 5.00 | 291.24 | 389.17 |
| EDARAVONE | 3.52 | 0.64 | 10.00 | 35.19 | 23.08 | 16.09 | 3.00 | 69.25 | 48.11 | 42.19 | 5.00 | 240.55 | 344.99 |
| FENAMISAL | 3.15 | 0.41 | 10.00 | 31.55 | 9.52 | 5.06 | 3.00 | 28.57 | 36.89 | 29.81 | 5.00 | 184.47 | 244.59 |
| 5-METHYL-7-METHOXYISOFLAVONE | 7.05 | 1.36 | 10.00 | 70.54 | 8.86 | 0.34 | 3.00 | 26.59 | 35.95 | 20.86 | 5.00 | 179.73 | 276.86 |
| LOSARTAN | 1.14 | 0.23 | 10.00 | 11.44 | 11.11 | 10.23 | 3.00 | 33.34 | 23.24 | 21.14 | 5.00 | 116.21 | 160.98 |
| CHLORMADINONE ACETATE | 0.66 | 0.24 | 10.00 | 6.61 | 10.09 | 0.60 | 3.00 | 30.26 | 16.79 | 1.26 | 5.00 | 83.96 | 120.83 |
| BIOCHANIN A | 1.24 | 0.08 | 10.00 | 12.38 | 3.53 | 3.39 | 3.00 | 10.58 | 7.96 | 7.15 | 5.00 | 39.79 | 62.75 |
| CYPROTERONE | 1.25 | 0.34 | 10.00 | 12.49 | 2.46 | 0.13 | 3.00 | 7.39 | 7.65 | 0.06 | 5.00 | 38.23 | 58.12 |
| OLMESARTAN | 0.79 | 0.17 | 10.00 | 7.88 | 4.06 | 3.08 | 3.00 | 12.19 | 6.49 | 4.47 | 5.00 | 32.44 | 52.50 |
| FLUOCINOLONE ACETONIDE | 0.59 | 0.17 | 10.00 | 5.89 | 2.40 | 0.13 | 3.00 | 7.21 | 5.95 | 0.48 | 5.00 | 29.74 | 42.83 |
| TRANDOLAPRIL | 2.84 | 1.24 | 10.00 | 28.35 | 6.06 | 5.44 | 3.00 | 18.17 | 5.86 | 5.11 | 5.00 | 29.31 | 75.83 |
| QUERCITRIN | 3.90 | 0.44 | 10.00 | 38.96 | 4.68 | 1.76 | 3.00 | 14.05 | 5.10 | 3.09 | 5.00 | 25.48 | 78.48 |
| URIDINE TRIPHOSPHATE TRISODIUM | 4.80 | 0.36 | 10.00 | 47.99 | 3.75 | 0.21 | 3.00 | 11.26 | 5.02 | 0.41 | 5.00 | 25.10 | 84.35 |
| DIPHENYLPYRALINE HYDROCHLORIDE | 3.65 | 1.35 | 10.00 | 36.46 | 3.10 | 2.01 | 3.00 | 9.29 | 4.90 | 3.23 | 5.00 | 24.52 | 70.27 |
| VERATRINE SULFATE | 3.23 | 0.81 | 10.00 | 32.33 | 4.09 | 0.17 | 3.00 | 12.27 | 4.82 | 0.27 | 5.00 | 24.11 | 68.72 |
| 3,5-DINITROCATECHOL | 5.55 | 1.35 | 10.00 | 55.47 | 4.74 | 0.39 | 3.00 | 14.22 | 4.76 | 0.19 | 5.00 | 23.78 | 93.48 |
| HYGROMYCIN B | 3.66 | 0.45 | 10.00 | 36.65 | 4.46 | 4.08 | 3.00 | 13.37 | 4.65 | 3.69 | 5.00 | 23.25 | 73.27 |
| ESCITALOPRAM OXALATE | 3.86 | 0.56 | 10.00 | 38.63 | 3.91 | 3.31 | 3.00 | 11.72 | 3.89 | 1.53 | 5.00 | 19.46 | 69.80 |
| DIAZOXIDE | 1.34 | 0.11 | 10.00 | 13.37 | 3.31 | 2.32 | 3.00 | 9.92 | 3.67 | 1.25 | 5.00 | 18.35 | 41.65 |
| ESTRADIOL | 1.85 | 0.61 | 10.00 | 18.47 | 1.53 | 0.34 | 3.00 | 4.59 | 2.75 | 1.10 | 5.00 | 13.76 | 36.83 |
| AMINOBENZTROPINE | 5.77 | 1.17 | 10.00 | 57.67 | 1.48 | * | 3.00 | 4.44 | 2.58 | * | 5.00 | 12.92 | 75.04 |
| DIACERIN | 1.28 | 0.36 | 10.00 | 12.76 | 0.19 | * | 3.00 | 0.58 | 2.56 | * | 5.00 | 12.81 | 26.16 |
| PERINDOPRIL ERBUMINE | 2.33 | 0.32 | 10.00 | 23.28 | 1.76 | 0.11 | 3.00 | 5.27 | 2.45 | 0.10 | 5.00 | 12.23 | 40.78 |
| PHENOLPHTHALEIN | 1.21 | 0.10 | 10.00 | 12.11 | 2.85 | 1.68 | 3.00 | 8.55 | 2.44 | 1.53 | 5.00 | 12.19 | 32.85 |
| ORPHENADRINE CITRATE | 1.08 | 0.39 | 10.00 | 10.84 | 0.41 | * | 3.00 | 1.24 | 1.66 | * | 5.00 | 8.29 | 20.36 |
| BECLOMETHASONE DIPROPIONATE | 0.48 | 0.07 | 10.00 | 4.78 | 1.06 | * | 3.00 | 3.17 | 1.55 | * | 5.00 | 7.74 | 15.69 |
| NIACIN | 0.77 | 0.21 | 10.00 | 7.75 | 0.70 | * | 3.00 | 2.11 | 1.35 | * | 5.00 | 6.73 | 16.58 |
| FLUDROCORTISONE ACETATE | 0.74 | 0.13 | 10.00 | 7.41 | 0.76 | * | 3.00 | 2.28 | 1.28 | * | 5.00 | 6.41 | 16.10 |
| AVOCADYNE ACETATE | 1.09 | 0.15 | 10.00 | 10.88 | 1.20 | * | 3.00 | 3.61 | 1.10 | * | 5.00 | 5.48 | 19.97 |
| KANAMYCIN A SULPHATE | 0.95 | 0.20 | 10.00 | 9.45 | 1.50 | * | 3.00 | 4.51 | 1.03 | * | 5.00 | 5.15 | 19.11 |
| LIDOCAINE HYDROCHLORIDE | 2.43 | 0.14 | 10.00 | 24.29 | 1.10 | * | 3.00 | 3.30 | 1.02 | * | 5.00 | 5.09 | 32.67 |
| S-(1,2-DICARBOXYETHYL)GLUTATHIONE | 1.37 | 0.15 | 10.00 | 13.66 | 0.71 | * | 3.00 | 2.12 | 0.85 | * | 5.00 | 4.23 | 20.01 |
| HYOSCYAMINE | 1.35 | 0.40 | 10.00 | 13.52 | 0.43 | * | 3.00 | 1.30 | 0.84 | * | 5.00 | 4.18 | 19.00 |
| VINPOCETINE | 0.83 | 0.26 | 10.00 | 8.31 | 0.96 | * | 3.00 | 2.89 | 0.62 | * | 5.00 | 3.11 | 14.31 |
| GENTAMICIN SULPHATE | 1.16 | 0.21 | 10.00 | 11.59 | 0.67 | * | 3.00 | 2.02 | 0.54 | * | 5.00 | 2.69 | 16.30 |
| ORLISTAT | 1.87 | 0.36 | 10.00 | 18.66 | 0.70 | * | 3.00 | 2.09 | 0.53 | * | 5.00 | 2.65 | 23.41 |
| ISOXSUPRINE | 1.20 | 0.40 | 10.00 | 12.00 | 0.95 | * | 3.00 | 2.84 | 0.49 | * | 5.00 | 2.46 | 17.31 |
| CINEOLE | 1.03 | 0.30 | 10.00 | 10.26 | 0.63 | * | 3.00 | 1.90 | 0.45 | * | 5.00 | 2.24 | 14.40 |
| TRIPELENNAMINE CITRATE | 3.61 | 0.92 | 10.00 | 36.08 | 0.21 | * | 3.00 | 0.63 | 0.22 | * | 5.00 | 1.10 | 37.81 |
| NYLIDRIN | 0.94 | 0.07 | 10.00 | 9.38 | 0.09 | * | 3.00 | 0.27 | 0.21 | * | 5.00 | 1.04 | 10.68 |
| ALPRENOLOL | 3.57 | 0.33 | 10.00 | 35.69 | 0.23 | * | 3.00 | 0.70 | 0.09 | * | 5.00 | 0.43 | 36.83 |
| HYDROXYTOLUIC ACID | 2.26 | 0.29 | 10.00 | 22.57 | 0.22 | * | 3.00 | 0.65 | 0.05 | * | 5.00 | 0.24 | 23.45 |
We assigned a different score to each experimental setting performed in purified OPC cultures, rewarding more late differentiation (Diff_MBP) than early differentiation (Diff_CGT) and both more than proliferation (Prolif; see “score assignment” in method section for details). The score contribution of each compound was derived by multiplying the assigned score to the raw data of the experimental setting it belongs to. Score contributions have been then summed per-compound (Total score). The asterisk indicates the drugs that have not achieved the established thresholds in the first gene expression analysis and were not tested again. The following positive controls were used: PDGF (20 ng/ml) for proliferation and T3 (30 ng/ml) and T4 (40 ng/ml) for differentiation assays.