Skip to main content
. 2017 Mar 31;12:571–581. doi: 10.1016/j.redox.2017.03.030

Fig. 1.

Fig. 1

Induction of rat MC lysis by rabbit serum. (A) Induction of MC lysis by rabbit serum. MCs were exposed to 10 μg/ml Thy-1 antibody in the presence of 5% native and heated-treated rabbit serum for 6 h. Cell morphology was photographed. (B) MCs were exposed to 10 μg/ml Thy-1 antibody in the presence of the indicated concentrations of rabbit serum for 6 h. Cellular supernatants were collected and assayed for LDH activities. Data shown are mean ±SE (n =4). ** P<0.01 vs. zero point control. (C) Calcein-AM/PI staining. Cells were either left untreated or treated with 5% rabbit serum in the absence or presence of 10 μg/ml anti-Thy-1 antibodies for 6 h. The viability of cells was determined using PI (red, dead cells) and calcein-AM (green, living cells) staining. (D) The percentage of PI-positive red cells per field in C. The number of PI-positive cells was counted. The data shown are mean ±SE (n =4). ** P<0.01 vs. zero control.