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. 2017 Mar 31;12:571–581. doi: 10.1016/j.redox.2017.03.030

Fig. 2.

Fig. 2.

GSH suppresses the rabbit serum-induced MC cell lysis. (A) Calcein-AM/PI staining of rabbit serum-treated cells. Cells were treated with the indicated concentrations of rabbit serum in the presence or absence of 3 mM GSH for 6 h. The viability of cells was determined using calcein (green) and PI (red) staining. (B, C) The percentage of calcein- and PI-positive cells in A. The number of calcein-positive green and PI positive red cells was counted and expressed as percentage of the total cells. Data shown are mean ±SE (n =4). ## P<0.01 vs. zero control; ** P<0.01 vs. respective control. (D) Determination of cell viability using WST assay. Cells were treated the same as above. Cell viability was evaluated by WST assay. Data shown are mean ±SE (n =4). ## P<0.01 vs. zero control; ** P<0.01 vs. respective control.