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. 2017 Apr 7;7:46238. doi: 10.1038/srep46238

Figure 2. Knockdown of CSE1L inhibits osteosarcoma cell growth in vitro.

Figure 2

(A,B) Levels of mRNA and protein expression were validated after CSE1L-specific siRNAs transfection in MNNG/HOS cells and U2OS cells by qRT-PCR and western blotting, respectively. Cell Counting Kit-8 (CCK-8) assays were performed after siRNA transfection. (C,D) Cell cycle assays were performed for CSE1L-silenced osteosarcoma cells and control cells. (E,F) Colony formation assays were performed for CSE1L-silenced osteosarcoma cells and control cells. Data are representative of results from three independent experiments. *P < 0.05. For western blotting, full-length gels are presented in Supplementary Figure S8.