Table 1.
List of proteins identified using the nano LC-ESI-Q-TOF with the specification of their NCBI accession number, name, score, molecular weight (MW) in Dalton (Da), isoelectric point (pI) and percentage sequence coverage. Proteins highlighted with asterisk (*) were not described before in Zebrafish embryo.
| Accession | Protein Name | Score | MW (Da) | pI | Coverage % |
|---|---|---|---|---|---|
| gi|166795887 | Vitellogenin 1 precursor | 1108 | 150308 | 8.68 | 19 |
| gi|94733730 | Vitellogenin 1 | 1039 | 149825 | 8.74 | 21 |
| gi|94733733 | Novel protein similar to vitellogenin 1 (vg1) | 913 | 149828 | 8.92 | 19 |
| gi|94733734 | Novel protein similar to vitellogenin 1 (vg1) | 835 | 150550 | 8.83 | 16 |
| gi|145337918 | Vtg1 protein | 780 | 116965 | 9.07 | 18 |
| gi|94733731 | Novel protein similar to vitellogenin 1 (vg1) | 762 | 149911 | 8.84 | 19 |
| gi|94732723 | Novel protein similar to vitellogenin 1 (vg1) | 745 | 147826 | 8.73 | 17 |
| gi|159155252* | Zgc:136383 protein | 720 | 124413 | 8.78 | 17 |
| gi|68448530 | Vitellogenin 5 | 559 | 149609 | 8.77 | 13 |
| gi|92097636 | Zgc:136383 | 402 | 28924 | 9.33 | 36 |
| gi|63100501 | Vtg1 protein | 345 | 36580 | 9.23 | 28 |
| gi|57864789 | Vitellogenin 7 | 341 | 24490 | 8.37 | 40 |
| gi|57864783 | Vitellogenin 4 | 334 | 31304 | 9.48 | 27 |
| gi|113678458 | Vitellogenin 2 isoform 1 precursor | 323 | 181208 | 8.70 | 11 |
| gi|125857991 | Zgc:136383 protein | 171 | 149328 | 8.93 | 9 |
| gi|15209312* | Procollagen type I alpha 2 chain | 169 | 147826 | 9.35 | 4 |
| gi|57864779 | Vitellogenin 2 | 122 | 69906 | 7.84 | 8 |
| gi|11118642 | Vitellogenin 3 precursor | 117 | 140477 | 6.92 | 2 |
| gi|303227889 | Vitellogenin 6 | 73 | 151677 | 8.84 | 4 |
| gi|13242157 * | Egg envelope protein ZP2 variant A | 71 | 48194 | 6.04 | 5 |
| gi|6644111 * | Nucleoside diphosphate kinase-Z1 | 69 | 17397 | 7.77 | 14 |
| gi|18859071* | Nucleoside diphosphate kinase 3 | 69 | 19558 | 7.68 | 7 |
| gi|126632622* | Novel protein containing a galactose binding Lectin domain | 67 | 19245 | 9.33 | 13 |
| gi|66773080 * | Mitochondrial ATP synthase beta subunit-like | 66 | 55080 | 5.25 | 4 |
| gi|38541767* | Ppia protein | 60 | 19745 | 9.30 | 13 |
| gi|1865782 | HSC70 protein | 58 | 71473 | 5.18 | 2 |
| gi|28279108 | Heat shock protein 8 | 58 | 71382 | 5.32 | 4 |
| gi|41152402* | Histone H2B 3 | 49 | 13940 | 10.31 | 11 |
| gi|41393113* | Collagen, type I, alpha 1b precursor | 46 | 137815 | 5.39 | 4 |
| gi|94732492 * | Ras homolog gene family, member F | 46 | 24035 | 9.00 | 6 |
| gi|47778620 * | Tryptophan hydroxylase D2 | 45 | 55686 | 6.56 | 1 |
| gi|68448517 * | Zona pellucida glycoprotein 3.2 precursor | 44 | 47365 | 4.92 | 2 |
| gi|326677766 * | PREDICTED: RIMS-binding protein 2-like | 41 | 138659 | 5.86 | 0 |
| gi|112419298 | Vtg3 protein | 40 | 60622 | 6.32 | 2 |
| gi|54400406 * | Glutaredoxin 3 | 39 | 36541 | 5.18 | 11 |
| gi|41152400* | Peptidylprolyl isomerase A, like | 37 | 17763 | 8.26 | 7 |