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. 2017 Feb 6;6(3):e1287247. doi: 10.1080/2162402X.2017.1287247

Figure 2.

Figure 2.

Increased foci coverage in PyMT/Il1r1−/− mice compared with PyMT mice at early time points. (A) Whole mounts of mammary fat pads of PyMT or PyMT/Il1r1−/− mice at 6 and 10 weeks of age were stained with hematoxylin and analyzed for foci coverage (n = 4–5 mice per time point per genotype, Student's t-test p < 0.001). (B) Representative pictures of whole mount of PyMT or PyMT/Il1r1−/− mice at 6 and 10 weeks of age. Boxed region represents insets. (C) H&E of mammary fat pad section of PyMT or PyMT/Il1r1−/− mice at 10 weeks of age. (D) Western blots depicting E-cadherin, Cyclin D1 and GAPDH expression in lysates from mammary fat pads of WT, Il1r1−/−, PyMT or PyMT/Il1r1−/− mice at 6 weeks of age. (E) Densitometry analysis of the ratios of E-cadherin/GAPDH and Cyclin D1/GAPDH, using the ImageJ software. (F) Mice were palpated twice a week to determine the first appearance of tumors. Tumor volume was measured weekly for the following 6 weeks (n = 8–12 mice per genotype, Student's t-test per time point).