Fig. 4.

Nutra II improves spontaneous locomotor activity in 5XFAD mice as determined using the open field task and elevated plus maze test - 5XFAD mice untreated exhibited reduced locomotor and exploratory activity in comparison with WT mice, as evidenced by reduced total distance traveled, average speed and rearing frequency in a novel environment (a). However, 5XFAD mice did not exhibit increased anxiety compared with WT mice, as determined by similar amounts of time in the central and peripheral zones (b). The hypoactivity observed in 5XFAD mice was reversed with Nutra II treatment (*P < 0.05 versus untreated). The reduced spontaneous locomotor activity in 5XFAD mice was confirmed by the reduced total distance traveled, average speed (c) and open, closed and total arms entries in elevated plus maze (d). This reduced activity was reversed with Nutra II treatment (**P < 0.01). Notably, 5XFAD mice did not exhibit increased anxiety, evidenced as similar proportion of time spent in the open and closed arms in comparison with WT mice.