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. 2017 Jan 6;6(3):e1278099. doi: 10.1080/2162402X.2016.1278099

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Figure 3. — BTN3A1 expression and subcellular localization in CRC cell lines. (A) BTN3A1 was evaluated in CRC cell lines by western blot. Immunoblot of cell lysates obtained from the indicated CRC cell lines as described in Materials and Methods, was probed with the anti-CD277 mAb (upper blot), or with a rabbit polyclonal anti-BTN3A1 antiserum (lower blot). β-actin was used as a loading control. (B) BTN3A1 localization in subcellular fractions (Cyt: cytosolic fraction, M: membrane-enriched fraction, N: nuclear fraction, Ck: cytoskeleton-enriched fraction) obtained with the Qproteome cell compartment kit from untreated o Zol-treated (10 µM for 24 h) DLD1 (left panel) or LS180 (right panel), as indicated. In each panel: β-tubulin as marker of Ck fractions, GAPDH for the Cyt/M fractions, lamin B for the N fraction.