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. 2017 Jan 6;6(3):e1278099. doi: 10.1080/2162402X.2016.1278099

Figure 5.

Figure 5.

Measurement of IPP produced by SW620, DLD1 or LS180 CRC cell lines upon exposure to Zol. Acetonitrile extracts from LS180 (A), DLD1 (B) and SW620 cells (C) untreated (d) or treated with 5 µM Zol for 24 h (a), or 50 µM (b) and 100 µM (c) Zol for 4 h and maintained in culture for additional 20 h, were analyzed by HPLC/TOF-MS. The relative abundance of the extracted ion current (EIC) (m/z 244.99 [M-H]-) for IPP/DMAPP (3,3-dimethylallyl pyrophosphate) is shown. LS180 cells were also exposed to 5 µM Zol for 4 h (A, a1), to test early effects of low-dose Zol, giving no appreciable increase of IPP over controls. Quantification of IPP produced upon exposure to either 5 µM Zol for 24 h (Aa, Ba, Ca) or 100 µM Zol for 4 h (Ac, Bc, Cc) in the indicated CRC cell lines. Data are shown as IPP pmol/mg of total pmol extracted by ACN/total protein content in cell lysates after ACN extraction.