Fig. 4. (a) Fluorescence microscopy images of co-cultured HCT116 and NCM460 cells stained with FITC-Annexin V and propidium iodide after incubation with 1 (2 μM) for 24 h. The NCM460 cells were pre-treated with blue-emissive CMF2HC dye for differentiation from HCT116 cells. The overlaid image was prepared using ImageJ, with apoptotic cells in green. Analysis by flow cytometry of the co-culture model of NCM460 cells, which were pre-treated by CMF2HC dye, and HCT116 cells after incubation with 1 (2 μM) for 24 h and (b) without and (c) with FITC-Annexin V staining.
