Fig. 5. Fluorescence microscopy images of co-cultured HCT116 and NCM460 cells stained with CellEvent™ Caspase-3/7 Green ReadyProbes Reagent after incubation with 1 (2 μM) for (a) 24 h and (b) 36 h, respectively. Flow cytometric analysis of the co-culture model stained with CellEvent™ Caspase-3/7 Green Ready Detection Reagent (2.5 μM) after incubation with (c–e) 1 (2 μM) and (f–h) Au1a (2 μM) for 24 h. The NCM460 cells were pre-treated with blue-emissive CMF2HC dye for differentiation from HCT116 cells. The overlaid images were prepared using ImageJ.
