Fig. 7. Evaluation of systemic toxicity in nude mice bearing HCT116 xenografts after treatment with 1 or 3. (a) H & E staining of lung, liver, kidney, heart and spleen sections from HCT116 xenografted nude mice (n = 8) at 24 days post-treatment. The black arrows in lung, liver and kidney sections denote sites of pulmonary hemorrhage, apoptotic cells and swollen epithelial cells, respectively. Blood biochemistry analysis of (b) ALT, (c) AST, (d) GLB, (e) BUN, (f) CREA, (g) UA, (h) LDH and (i) CK in the mice (n = 8) at 24 days post-treatment. Bars with different characters were statistically significant at p < 0.05 level.