Table 3.
Reproductive Maturation and Anogenital Distance.
| Control
|
Bisphenol A
|
17α-Ethinyl Estradiol
|
||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| 0.0 ppm | 0.03 ppm | 0.3 ppm | 3.0 ppm | 30 ppm | 300 ppm | 0.0001 ppm | 0.001 ppm | 0.01 ppm | ||
| BPS | 29.5 ± 1.9 | 27.6 ± 0.8a | 28.4 ± 1.5 | 27.8 ± 0.8 a | 28.1 ± 0.6 | 28.1 ± 0.6 | 28.2 ± 0.6 | 27.2 ± 1.4a | 28.5 ± 0.8 | |
| VO | 25.5 ± 2.7 | 26.1 ± 2.6 | 26.9 ± 2.2 | 26.9 ± 2.3 | 26.1 ± 2.2 | 26.2 ± 2.8 | 26.7 ± 3.0 | 26.5 ± 1.7 | 25.8 ± 1.4 | |
| Cornification | 29.7 ± 3.0 | 30.5 ± 2.1 | 31.1 ± 2.4 | 30.1 ± 3.2 | 30.5 ± 1.1 | 30.1 ± 1.7 | 31.0 ± 3.3 | 29.9 ± 2.3 | 29.8 ± 2.3 | |
| AGD (mm) | ||||||||||
| PND 14 | F | 5.34 ± 0.52 | 5.42 ± 0.43 | 5.65 ± 0.72 | 5.66 ± 0.72 | 5.99 ± 0.61 | 5.89 ± 0.72 | 5.84 ± 0.63 | 5.63 ± 0.59 | 5.88 ± 0.60 |
| M | 6.86 ± 0.56 | 7.13 ± 0.60 | 6.92 ± 0.69 | 7.16 ± 0.79 | 7.44 ± 0.63 | 7.43 ± 0.70 | 7.45 ± 0.52 | 7.07 ± 0.46 | 7.53 ± 0.79 | |
| PND 21 | F | 7.31 ± 0.94 | 7.65 ± 0.54 | 7.39 ± 0.86 | 7.44 ± 0.57 | 7.80 ± 0.52 | 7.69 ± 0.53 | 8.00 ± 5.89 | 7.81 ± 0.69 | 7.74 ± 0.75 |
| M | 10.24 ± 1.29 | 11.27 ± 0.91a | 10.88 ± 1.01 | 11.12 ± 0.96 | 11.36 ± 1.14a | 11.48 ± 0.99a | 11.57 ± 0.96a | 11.44 ± 1.17a | 11.19 ± 1.57 | |
Abbreviations: BPS, postnatal day of balanopreputial separation; VO, postnatal day of vaginal opening; cornification, postnatal day of first vaginal cornification; AGD, anogenital distance; ANCOVA, analysis of covariance; ANOVA, analysis of variance; SD, standard deviation.
Note: Values represent group mean ± SD (n ≥ 16 litters).
a
Significantly different from control by Kaplan-Meier (BPS, VO, and cornification) or ANOVA (AGD) analysis (P < 0.05).