Table 2.
In Vitro Studies Using Biofilms to Form Secondary Caries around Dental Composite Restorations to Study the Effect of Gap Size.
| Study | Gap Size, µm | Biofilm | Time, d | Gap Effect? |
|---|---|---|---|---|
| Totiam et al. (2007) | 0, 25, 250, 1,000 | Streptococcus mutans | 8 | Yes |
| Cenci et al. (2009) | 0, 50, 100, 180, 250 | Microcosm | 18 | Yes |
| Diercke et al. (2009) | 50, 100, 250 | S. mutans | 21 | Yes |
| Hayati et al. (2011) | Bond–no bond | S. mutans, Streptococcus sobrinus, Streptococcus gordonii | 30 | Yes |
| Nassar and González-Cabezas (2011) | 30, 525, 1000 | S. mutans | 8 | Yes |
| Kuper, van de Sande, et al. (2015) | 200 to 400 | Microcosm | 20 | Yes |