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. 2016 Oct 4;242(3):275–285. doi: 10.1177/1535370216669839

Figure 4.

Figure 4

Knockdown of VEGF reduced the cell viability, induced cell apoptosis, and reduced the cell invasion and migration ability in HKF. (a) Expression level of p-Akt, Akt, fibrous protein, and α-SMA treated with siVEGF. (b) Representative images of transwell assays, knockdown of VEGF reduced the invasion and migration ability of HKF. (c) Representative images of cell apoptosis as detected by flow cytometry, knockdown of VEGF induced cell apoptosis in HKF. Quantitative analysis results of VEGF knockdown efficiency as detected by RT-qPCR, level of VEGF mRNA was significantly reduced after transfection of siVEGF. (d) Representative images of cell cycle distribution as detected by flow cytometry, knockdown of VEGF had no impact on the cell cycle distribution in HKF. (e) Quantitative analysis results of CCK-8 assay, knockdown of VEGF inhibited the cell proliferation in HKF. (f) Overexpression of miR-205-5p-induced relative protein downregulation. “*”, P < 0.05 compared with NC group. “***”, P < 0.001 compared with NC group. (A color version of this figure is available in the online journal.)