Figure 6.

Reduction of PKC protein expression and inhibition of PKC activity through Baccharis trimera extract and quercetin. (a) Densitometric scanning of PKC after normalization with β-actin. Results of the Western blotting assay showing PKC activation through PMA + Ionomycin in SK Hep-1 cells and the effect of Baccharis trimera hydroethanolic extract and quercetin on this protein. *P < 0.05 for values significantly different from C (PMA + iono). The analyses were performed using Quantity One image analysis software (Bio Rad). (b) PKC activity in cells exposed to Baccharis trimera extract and quercetin, followed by stimulation with PMA + ionomycin was commercially assessed using a kit purchased from Enzo Life Sciences. As a positive control, PKC was supplied in the kit. ^#P < 0.0001 for values significantly different from C (control cells); **P < 0.001 and ***P < 0.0001 for values significantly different from C (PMA + Iono). The results are expressed as the means ± SEM. Statistically significant differences were determined using One-way ANOVA and Dunnett’s post-test. These analyses were performed in triplicate