Table 1.
Primers used in this study.
| Gene | Primer sequence | Size (bp) | Application |
|---|---|---|---|
| IGF1 | CTCCTCGCATCTCTTCTACC | 205 | In situ hybridization |
| GCAGCACTCATCCACTATTC | |||
| IRS1 | CAGTGGGGACTACATGCC | 307 | In situ hybridization |
| ACCTGTACAAGACAGGAGTTTG | |||
| IRS2 | GACCTGCGGGTCTACTG | 208 | In situ hybridization |
| GCGAGTAAGTCCGCTTC | |||
| RUNX1 | CCTTGAACCACTCCACTGCC | 268 | In situ hybridization |
| GACGGCAGAGTAG GGAACTG | |||
| IGF1 | CCTCGCATCTCTTCTACCTGG | 191 | Real time PCR |
| CACTATTCCCGTCTGGGGC | |||
| IHH | AGGAGTCGCTGCATTACGAA | 152 | Real time PCR |
| CTTGACGGAGCAATGCACG | |||
| IRS1 | CGGGTGAACCTCAGTCCCAA | 212 | Real time PCR |
| GAGCTGTGGCGTTTGACATC | |||
| IRS2 | CCAACGGGGACTACCTCAAC | 146 | Real time PCR |
| GTGAGGGGCCTTGTAGGAG | |||
| RUNX1 | CTTCACAAACCCACCGCAAG | 132 | Real time PCR |
| TCGGAAAAGGACAAGCTCCC | |||
| Gapdh | GAAGGGTGGCGCCAAGAGGG | 142 | Real time PCR |
| GGGGGCCAAGCAGTTGGTGG |